Melanin deficiency is rare among vertebrates, and chromatic disorders in bats are considered exceptional. Among these, albinism is a hereditary condition caused by mutations affecting melanin synthesis, resulting in white fur and red eyes. Although albinism has been documented in several bat families around the world, records for the Lesser horseshoe bat (Rhinolophus hipposideros) remain scarce and mostly confined to Central Europe. We report the first documented case of albinism in R. hipposideros in Italy, observed in 2025 along the Adriatic coast of the province of Lecce. The albino individual, a lactating female with red eyes and completely white fur, was found within a nursery colony of approximately 172 individuals roosting in an artificial cavity. The bat exhibited normal behaviour and was observed carrying a normally pigmented pup, suggesting possible successful reproduction and apparent social integration based on the available observations. This observation represents one of the southernmost records of albinism in lesser horseshoe bat and provides rare evidence that this condition does not necessarily impair reproductive activity or social behaviour under sheltered roosting conditions. Further studies are needed to assess the potential effects of albinism on fitness and survival in bats.