The knowledge of morphological features of species can help to understand other related biological aspects. In Italy the European pine marten Martes martes seems to show a recent expansion of its distribution, however information on this species in our Country are scarce. Very few data on biometric measurements are available, mainly referred to the Sardinian population, and the only published study on peninsular populations was based exclusively on cranial morphology. For this reasons we aimed to provide external morphological data on thirty-three pine marten in Tuscany, central Italy. In addition, we found that pine marten appear to be quite distinguishable from the sibling species stone marten M. foina by inspection of coat colour and marking pattern, showing that qualitative diagnosis of external morphological traits can be very useful to identify this species in central Italy. In fact, genetic analyses on samples of hair confirmed the correct attribution.