Nature-based tourism is often promoted as compatible with biodiversity conservation, yet its physiological correlates in wildlife remain incompletely understood. Protected areas can limit direct exploitation and habitat alteration without eliminating non-lethal human presence. We measured faecal cortisol metabolite (FCM) concentrations in four ungulate species across high- and low-tourism periods in Bieszczady National Park and managed forests. We examined seasonal differences and associations with daily visitor numbers, distance from forest roads or tourist trails, management designation, and sex. FCM concentrations were higher during the high-tourism season in European bison, wild boar, and roe deer (approximately 2.3-fold, 2.9-fold, and 2.0-fold, respectively). In red deer, FCM concentrations were approximately 27% lower, but this contrast was not statistically detectable. We detected no statistically detectable associations with visitor number, distance, or management designation, although the management comparison was not replicated at the landscape level. Because season also covaried with environmental conditions, reproductive stage, and hunting pressure, these seasonal associations cannot be attributed specifically to tourism. The results illustrate species-specific seasonal variation and support combining physiological indicators with conventional wildlife monitoring; they also motivate further studies integrating visitor exposure, environmental covariates, and management interventions.